Journal: Communications Biology

Article Title: Retinoic acid receptor alpha inhibits ferroptosis by promoting thioredoxin and protein phosphatase 1F in lung adenocarcinoma

doi: 10.1038/s42003-024-06452-7

Figure Lengend Snippet: a CCK8 assays to detect the cell viability of A549 and PC9 cells treated with RSL3 (2 μM), RSL3 (2 μM) plus DFO (100 μM) or fer-1 (20 μM) for 24 h after incubating with DMSO, Ch55 (5 μM), AM580 (5 μM) ATRA (20 μM) or AGN193109 (5 μM) for 24 h. ( n = 4 biologically independent experiments, Student t -test). b – e MDA level ( b ) lipid peroxidation ( c ), mitochondrial membrane potential (MMP) by TMRE ( d ), and labile iron pool (LIP) by CA-AM ( e ) were accessed in A549 and PC9 cells treated with DMSO or RSL3 (1 μM) for 24 h after incubating with DMSO, Ch55 (5 μM), AM580 (5 μM) ATRA (20 μM) or AGN193109 (5 μM) for 24 h. ( n = 3 biologically independent experiments, Student t -test). f Light microscopy images showed the degrees of “ballooning phenotype” in A549 and PC9 cells treated with DMSO or RSL3 (1 μM) for 24 h after incubating with DMSO, Ch55 (5 μM), AM580 (5 μM) ATRA (20 μM) or AGN193109 (5 μM) for 24 h. Scale bar:50 μm. The zoomed-in figures, scale bar: 250 μm. ns, not significant; * p < 0.05; ** p < 0.01; *** p < 0.001; **** p < 0.0001.

Article Snippet: The RSL3, Imidazole Ketone Erastin (IKE), Erastin, Ferric ammonium citrate (FAC), RARA inhibitor AGN193109, RARA activator Ch55 and AM580, PAK inhibitor FRAX486, cisplatin (CDDP), and pemetrexed were acquired from Topscience (Shanghai, China) and were dissolved in dimethyl sulfoxide (DMSO).

Techniques: Membrane, Light Microscopy

Journal: Communications Biology

Article Title: Retinoic acid receptor alpha inhibits ferroptosis by promoting thioredoxin and protein phosphatase 1F in lung adenocarcinoma

doi: 10.1038/s42003-024-06452-7

Figure Lengend Snippet: a qRT-PCR and WB assays confirmed the mRNA and protein expression level of RARA in A549 and PC9 cells after transfection with NC (control shRNA), RARA-SH1, or RARA-SH2 lentivirus. ( n = 3 biologically independent experiments, Student t -test) ( b ) CCK8 assays to detect the cell viability of NC, RARA-SH1, or RARA-SH2 groups in A549 and PC9 cells treated with a gradient dose of RSL3 or IKE for 24 h. ( n = 4 biologically independent experiments, two-way ANOVA) ( c ) CCK8 assays to detect the cell viability of NC, RARA-SH1, or RARA-SH2 groups in A549 and PC9 cells treated with DMSO or RSL3 (2 μM) for 24 h after incubating with DMSO, Ch55 (5 μM), AM580 (5 μM) ATRA (20 μM) or AGN193109 (5 μM) for 24 h. ( n = 4 biologically independent experiments, Student t -test). d – g MDA level ( d ), lipid peroxidation ( e ), MMP by TMRE ( g ) and LIP by CA-AM ( g ) were accessed in NC, RARA-SH1 groups of A549 and PC9 cells treated with DMSO or RSL3 (1 μM) for 24 h after incubating with DMSO, Ch55 (5 μM), AM580 (5 μM) ATRA (20 μM) or AGN193109 (5 μM) for 24 h. ( n = 3 biologically independent experiments, Student t -test) ns, not significant * p < 0.05; ** p < 0.01; *** p < 0.001;**** p < 0.0001.

Article Snippet: The RSL3, Imidazole Ketone Erastin (IKE), Erastin, Ferric ammonium citrate (FAC), RARA inhibitor AGN193109, RARA activator Ch55 and AM580, PAK inhibitor FRAX486, cisplatin (CDDP), and pemetrexed were acquired from Topscience (Shanghai, China) and were dissolved in dimethyl sulfoxide (DMSO).

Techniques: Quantitative RT-PCR, Expressing, Transfection, Control, shRNA